Response of mouse haemopoietic colony formers to acute or continuous gamma irradiation

Journal Article · Tue May 01 00:00:00 EDT 1973 · Biomedicine, v. 19, no. 5, pp. 183-186

OSTI ID:4424155

Testa, N G; Hendry, J H; Lajtha, L G

The effect of single doses of/sup 137/Cs gamma rays or continuous /sup 60/Co gamma rays on mouse hemopoietic colony-forming units (CFU) was investigated using both the in vivo spleen colony assay technique (CFU/sub s/,) and the in vitro colony assay technique (CFU/sub c/). The difference in the respective D/sub o/ slopes was maintained during continuous irradiation. At the dose rate used (3.2 rad/hr) the shoulder on the CFU/sub s/ survival curve was abolished as expected, but the slopes of the early portions of the survival curves for both CFU/sub s/, and CFU/sub c/ were similar to those obtained after acute irradiation (600 rad/ min). A CFU/sub c/ subpopulation, which appears after adding red blood cells to the culture, showed a similar acute radiosensitivity to the whole CFU/sub c/ population but was less depleted under continuous irradiation. The results are discussed in relation to the kinetics of progenitor hemopoietic cell populations after a single, or during a prolonged, stress. (FR)

• Research Organization: Christie Hospital and Holt Radium Inst., Manchester, Eng.
• NSA Number: NSA-29-000534
• OSTI ID: 4424155
• Journal Information: Biomedicine, v. 19, no. 5, pp. 183-186, Other Information: Orig. Receipt Date: 30-JUN-74
• Country of Publication: France
• Language: English

Related Subjects

N48210* -Life Sciences-Radiation Effects on Cells- External Source
*CFU- BIOLOGICAL RADIATION EFFECTS
*HEMATOPOIETIC SYSTEM- CFU
ACUTE IRRADIATION
BONE MARROW
CELL CULTURES
CELL PROLIFERATION
CHRONIC IRRADIATION
DOSE RATES
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
MICE
PHOTON BEAMS
RADIATION DOSES
SPLEEN COLONY FORMATION
STEM CELLS
SURVIVAL CURVES