Corynebacterium parvum-induced radiosensitivity and cycling changes of hematopoietic spleen colony-forming units

Journal Article · Fri Jul 01 00:00:00 EDT 1977 · J. Natl. Cancer Inst.; (United States)

OSTI ID:7302781

Maruyama, Y; Magura, C; Feola, J

Ten days after total-body irradiation with 550 rads of /sup 60/Co, spleen colonies were observed in adult C57BL mice. A change in radiosensitivity induced by Corynebacterium parvum, as measured by increased numbers of colony-forming units that survived the 550 rads, began shortly after C. parvum stimulation and extended for at least 7 days before irradiation. C. parvum given 4-24 hours before, followed by high specific activity (/sup 3/H)thymidine (HSATT) 1 hour before total-body irradiation greatly reduced survival of the stem cells that formed spleen colonies (CFU/sub s/) and CFU/sub s/ radiosensitivity to control levels. The HSATT sensitivity by ''suicide'' assay in vivo and the time-response change in radiosensitivity corresponded with the decrease in radiosensitivity, which showed that CFU/sub s/ were stimulated by C. parvum administration and entered the S-phase shortly after stimulation. The data indicated a resting population close to the S-phase. After stimulation, this population entered S-phase. Syngeneic mouse lymphoma cells injected iv 24 hours earlier did not elicit any effect as a stimulus to CFU/sub s/ radiosensitivity change.

• Research Organization: Univ. of Kentucky, Lexington
• OSTI ID: 7302781
• Journal Information: J. Natl. Cancer Inst.; (United States), Vol. 59:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BLOOD FORMATION
BIOLOGICAL RADIATION EFFECTS
CFU
SPLEEN
STEM CELLS
RADIOSENSITIVITY
CELL CYCLE
GAMMA RADIATION
MICE
THYMIDINE
TRITIUM
TUMOR CELLS
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
IONIZING RADIATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RIBOSIDES
RODENTS
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals