Dose rate effects on the survival of normal hematopoietic stem cells cells of BALB/c mice

Journal Article · Fri Apr 01 04:00:00 EST 1983 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(83)90075-5· OSTI ID:5391276

Glasgow, G P ^[1]; Beetham, K L; Mill, W B

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1. Washington Univ. School of Medicine, St. Louis, MO

The use of total body irradiation (TBI) to ablate malignant stem cells in leukemia patients prior to bone marrow transplantation and the use of hemibody irradiation (HBI) for treating osseous metastases have focused attention on the dose rate effects, if any, exhibited by normal or malignant hematopoietic stem cells. Using male BALB/c mice 10 to 12 weeks old, we investigated dose rate effects at 103, 45 and 8 rad/ min over the dose range from 100 to 500 rad. Bone marrow cells from the femurs of irradiated donor mice were transplanted into lethally irradiated (720 rad) mice of the same age, sex, and strain. Recipient mice were sacrifices 9 days later, their spleens fixed, stained with Bouin's solution, and the macroscopic colonies counted to determine the number of colony forming units (CFU) per femur. Surviving fractions were determined by comparisons to the CFU's of non-treated controls. The logarithms of the surviving fractions, S, versus dose, D, (in rad) were least squares fitted and the extrapolation number, n, and D/sub 0/ obtained. The extrapolation numbers ranged from 0.65+/-0.15 to 0.81, and D/sub 0/ ranged from 61.7+/- 3.4 to 69.0+/- 2.8. There are no statistically significant differences between the n's and /d/sub 0/'s for these different dose rates over the dose range from 100 to 500 rad, as measured by spleen CFU assay of normal femoral marrow. The D's are appropriate for this radiosensitive mouse strain. These data are compared to those from other studies using the same method of CFU assay.

• OSTI ID: 5391276
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 9:4; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW CELLS
CFU
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DOSE RATES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
HARD X RADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MICE
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION