Radiation response of human normal and leukemic hemopoietic cells assayed by in vitro colony formation

Journal Article · Mon Apr 01 00:00:00 EST 1985 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(85)90315-3· OSTI ID:5444913

Kimler, B F; Park, C H; Yakar, D; Mies, R M

The effect of ionizing radiation on the survival of bone marrow cells from patients with acute nonlymphocytic leukemia or from hematologically normal controls was studied using colony formation as an endpoint. A modified agar culture method which incorporated daily feeding with new medium was used to allow the growth of leukemic cell colonies. Analysis of radiation-dose survival curves revealed that normal bone marrow cell populations exhibited a relatively steep slope. There was essentially no shoulder to the survival curves. The leukemic cells tested displayed survival curves that did not differ qualitatively from those obtained with normal cells, i.e., steep slopes and negligible shoulders. However, the average value of the D/sub 0/ was statistically different than that obtained for the normal cells. The results of these studies may have implications both for the use of radiation therapy for the treatment of malignant hemopoietic diseases, and for total body irradiation prior to bone marrow transplantation.

• Research Organization: Univ. of Kansas Medical Center, Kansas City
• OSTI ID: 5444913
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 11:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
TUMOR CELLS
CESIUM 137
COBALT 60
COLONY FORMATION
EXPERIMENTAL DATA
GAMMA RADIATION
IN VITRO
LEUKEMIA
PATIENTS
RADIOTHERAPY
STEM CELLS
SURVIVAL CURVES
X RADIATION
ALKALI METAL ISOTOPES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CESIUM ISOTOPES
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DISEASES
ELECTROMAGNETIC RADIATION
HEMIC DISEASES
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MEDICINE
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOLOGY
SOMATIC CELLS
THERAPY
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)