Transformation of hamster embryo fibroblasts by herpes simplex viruses Type 1 and Type 2
Hamster embryo fibroblast cells can be transformed aftcr infection by ultraviolet light-irradiated herpes simplex viruses type 1 (HSV-1) or type 2 (HSV- 2). Two of 12 strains of HSV-1 and 7 of 15 strains of HSV-2 induced cell transformation. Transformed cells from two strains of HSV-2 and one strain of HSV-1 were tested for oncogenicity in newborn Syrian hamsters. One cell line transformed by HSV-2 strain 333 was found to be oncogenic. The remaining two cell lines were not oncogenic. Herpesvirus antigens were found in the cytoplasm and on the surface of the transformed cells. Neutralizing antibodies were produced in hamsters developing tumors after the injection of the HSV-2- transformed cells. Weanling hamsters that were preimmunized by injection of HSV- 1 or HSV-2 did not develop transplantation immunity to the HSV-2transformed cells as a result of this preimmunization. HSV-2transformed cells metastasized after injection into hamsters. The number of metastases was not inhibited by HSV-1 or HSV-2 immunization but was actually enhanced. Immunization of hamsters with simian virus 40 inhibited metastases but did not reduce the rate of primary tumor development. (auth)
- Research Organization:
- Pennsylvania State Univ., Hershey
- NSA Number:
- NSA-29-005297
- OSTI ID:
- 4406581
- Journal Information:
- Cancer Res., v. 33, pp. 1527-1534, Journal Name: Cancer Res., v. 33, pp. 1527-1534; ISSN CNREA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Quantitative assay for transformation of 3T3 cells by herpes simplex virus type 2
Herpes simplex virus induces the replication of foreign DNA