Herpes simplex virus induces the replication of foreign DNA
Plasmids containing the simian virus 40 (SV40) DNA replication origin and the large T gene are replicated in Vero monkey cells but not in rabbit skin cells. Efficient replication of the plasmids was observed in rabbit cells infected with herpes simplex virus type 1 (HSV-1) and HSV-2. The HSV-induced replication required the large T antigen and the SV40 replication origin. However, it produced concatemeric molecules resembling replicative intermediates of HSV DNA and was sensitive to phosphonoacetate at concentrations known to inhibit the HSV DNA polymerase. Therefore, it involved the HSV DNA polymerase itself or a viral gene product(s) which was expressed following the replication of HSV DNA. Analyses of test plasmids lacking SV40 or HSV DNA sequences showed that, under some conditions. HSV also induced low-level replication of test plasmids containing no known eucaryotic replication origins. Together, these results show that HSV induces a DNA replicative activity which amplifies foreign DNA. The relevance of these findings to the putative transforming potential of HSV is discussed.
- Research Organization:
- Dept. of Molecular Genetics and Cell Biology, The Univ. of Chicago, Chicago, IL (US)
- OSTI ID:
- 6703519
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:8; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CELL CULTURES
CELL TRANSFORMATIONS
DISEASES
DNA POLYMERASES
DNA REPLICATION
ENZYME ACTIVITY
ENZYMES
GENE AMPLIFICATION
GENE RECOMBINATION PROTEINS
HERPES SIMPLEX
INFECTIOUS DISEASES
KINETICS
MAMMALS
MICROORGANISMS
MOLECULAR BIOLOGY
MONKEYS
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PARASITES
PHENOTYPE
PHOSPHORUS-GROUP TRANSFERASES
PLASMIDS
POLYMERASES
PRIMATES
PROTEINS
RABBITS
REACTION KINETICS
SIMIAN VIRUS
SKIN DISEASES
TRANSFERASES
VERTEBRATES
VIRAL DISEASES
VIRUSES