Repair of DNA following incorporation of 1-beta-D-arabinofuranosylcytosine into herpes simplex virus type 1

Journal Article · Tue May 01 04:00:00 EDT 1984 · Cancer Res.; (United States)

OSTI ID:6782676

Bubley, G J; Crumpacker, C S; Schnipper, L E

The nucleoside analogue 1-beta-D-arabinofuranosylcytosine (ara-C) is incorporated into herpes simplex virus type 1 (HSV-1) DNA, and this correlates with inhibition of virus replication. The technique of Weigle-type reactivation (WR) was used to compare the ability of induced cellular DNA repair pathways to recognize or repair ara-C incorporated into HSV-1 DNA and ultraviolet (UV)-irradiated virus DNA (254 nm). Pretreatment of monkey cells with low-fluence UV irradiation, growth in cis-dichlorodiammineplatinum(II), or growth in ara-C followed by infection after a 24-hr incubation period resulted in enhanced survival of UV-irradiated HSV-1. Under the same experimental conditions, no reactivation of HSV-1 inactivated by growth in ara-C is observed. Comparisons between uninfected Vero cells exposed to UV irradiation (30 J/m2) or grown in 10(-6) M ara-C demonstrated repair replication in irradiated cells, whereas there was no evidence for DNA repair at various time intervals following removal of the nucleoside analogue. These observations suggest that, once ara-C is incorporated into HSV-1 or eukaryotic DNA, it is not recognized as a repairable lesion within the limits of the DNA repair assays used in these studies.

• Research Organization: Charles A. Dana Research Institute, Harvard-Thorndike Laboratory of Beth Israel Hospital, Boston, Massachusetts
• OSTI ID: 6782676
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44:5; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
DNA REPAIR
ELECTROMAGNETIC RADIATION
HERPES SIMPLEX
INFECTIOUS DISEASES
KINETICS
MAMMALS
MONKEYS
PLATINUM COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
SKIN DISEASES
TRANSITION ELEMENT COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES