Enhanced replication of herpes simplex virus type 1 in human cells

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Journal of Dental Research; (USA)

DOI:https://doi.org/10.1177/00220345910700020301· OSTI ID:6053854

Miller, C S; Smith, K O ^[1]

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1. Univ. of Kentucky College of Dentistry, Lexington (USA)

The effects of DNA-damaging agents on the replication of herpes simplex virus type 1 (HSV-1) were assessed in vitro. Monolayers of human lung fibroblast cell lines were exposed to DNA-damaging agents (methyl methanesulfonate (MMS), methyl methanethiosulfonate (MMTS), ultraviolet light (UV), or gamma radiation (GR)) at specific intervals, before or after inoculation with low levels of HSV-1. The ability of cell monolayers to support HSV-1 replication was measured by direct plaque assay and was compared with that of untreated control samples. In this system, monolayers of different cell lines infected with identical HSV-1 strains demonstrated dissimilar levels of recovery of the infectious virus. Exposure of DNA-repair-competent cell cultures to DNA-damaging agents produced time-dependent enhanced virus replication. Treatment with agent before virus inoculation significantly (p less than 0.025) increased the number of plaques by 10 to 68%, compared with untreated control cultures, while treatment with agent after virus adsorption significantly increased (p less than 0.025) the number of plaques by 7 to 15%. In a parallel series of experiments, cells deficient in DNA repair (xeroderma pigmentosum) failed to support enhanced virus replication. These results suggest that after exposure to DNA-damaging agents, fibroblasts competent in DNA repair amplify the replication of HSV-1, and that DNA-repair mechanisms that act on a variety of chromosomal lesions may be involved in the repair and biological activation of HSV-1 genomes.

• OSTI ID: 6053854
• Journal Information: Journal of Dental Research; (USA), Journal Name: Journal of Dental Research; (USA) Vol. 70:2; ISSN 0022-0345; ISSN JDREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DISEASES
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ESTERS
FIBROBLASTS
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HERPES SIMPLEX
INFECTIOUS DISEASES
IONIZING RADIATIONS
MAMMALS
MAN
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES
XP CELLS