Involvement of DNA polymerase alpha in host cell reactivation of UV-irradiated herpes simplex virus

Journal Article · Wed Feb 01 04:00:00 EST 1984 · J. Virol.; (United States)

OSTI ID:6748637

Nishiyama, Y; Yoshida, S; Maeno, K

Aphidicolin is a potent inhibitor of both host cell DNA polymerase alpha and herpes simplex virus (HSV)-induced DNA polymerase but has no effect on DNA polymerases beta and gamma of host cells. By using an aphidicolin-resistant mutant (Aphr) of HSV, a possible involvement of DNA polymerase alpha in host cell reactivation of UV-damaged HSV was studied. Plaque formation by UV-irradiated Aphr was markedly inhibited by 1 microgram of aphidicolin per ml, which did not affect the plating efficiency of nonirradiated Aphr. Aphidicolin added before 12 h postinfection inhibited plaque formation by irradiated Aphr, which became aphidicolin insensitive after 36 h postinfection. The results strongly suggest that host cell DNA polymerase alpha is involved in the repair of UV-irradiated HSV DNA.

• Research Organization: Nagoya Univ. School of Medicine, Japan
• OSTI ID: 6748637
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 49:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HERPES SIMPLEX
HOST-CELL REACTIVATION
INFECTIOUS DISEASES
INHIBITION
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
PLAQUE FORMATION
POLYMERASES
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
TRANSFERASES
ULTRAVIOLET RADIATION
VIRAL DISEASES