Specific inactivation of cytotoxic lymphocytes after adsorption of $sup 51$Cr-labeled fibroblast monolayers

Journal Article · Sat Dec 01 04:00:00 EST 1973 · Transplantation, v. 16, no. 6, pp. 579-582

OSTI ID:4365180

Ritter, J; Lohmann-Matthes, M L; Fischer, H

Immune C57BL anti-DBA/2 spleen cells are incubated on DBA/2 fibroblast monolayers that were labeled to a high activity with /sup 51/Cr, After 90 min of incubation their cytotoxic potential is only slightly decreased as compared to immune lymphocytes adsorbed on unlabeled DBA/2 or nonrelevant C57AL monolayers. However, 18 hr later their cytotoxic potential is almost completely abolished, whereas in control situations the sensitized lymphocytes retain their full cytotoxic capacity. The reaction seems to be specific. The recovered cells can be sensitized against third party cells in a graft-versus-host reaction. Furthermore, the recovered cells are capable of restoring lethally irradiated C57BL mice as measured by the plaque-forming cell response to sheep red blood cells. The specific elimination of cytotoxic lymphocytes in this system is mainly performed by the K/sub alpha / radiation of /sup 51/Cr, which leads to in terphase death of the radiosensitive cytotoxic lymphocytes. (auth)

• Research Organization: Max-Planck Institut fuer Immunobiologie, Freiburg i. B.
• NSA Number: NSA-29-008024
• OSTI ID: 4365180
• Journal Information: Transplantation, v. 16, no. 6, pp. 579-582, Journal Name: Transplantation, v. 16, no. 6, pp. 579-582; ISSN TRPLA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*LYMPHOCYTES-- BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
CHROMIUM 51
FIBROBLASTS
GRAFT-HOST REACTION
INACTIVATION
LABELLED COMPOUNDS
LETHAL IRRADIATION
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
SPLEEN CELLS SPLEEN CELLS