Characteristics of ovine cytotoxic lymphocytes
Thesis/Dissertation
·
OSTI ID:6859756
Experiments were conducted to examine characteristics of the effector cells responsible for cell-mediated cytotoxicity in the sheep. Conditions for the production and assay of ovine T cell growth factor (TCGF) activity were evaluated. Peripheral blood leukocytes (PBL) were stimulated with concanavalin A (Con A) in the presence of 2% autologous serum or serum-free media. A 28 h proliferation assay with 2.5 x 10/sup 4/ h Con A blasts per well was optimal for detection of TCGF. Peak TCGF activity occurred with a 30-37kD molecular weight fraction. Ovine PBL were used for in vitro generation of genetically-restricted cytotoxic T lymphocytes (CTL). Peripheral blood leukocytes from sheep that had been previously inoculated with live vaccinia virus were stimulated by being cultured in vitro on glutaraldehyde-fixed vaccinia-infected autologous skin fibroblasts. Cytotoxic T lymphocyte activity was assessed in a 6 h /sup 51/Cr-release assay on autologous and allogeneic fibroblasts targets. Killing was restricted to virus-infected autologous targets. In vitro generation of both anti-vaccinia and anti-TNP CTL activity could be enhanced by the addition of TCGF containing media from ConA-stimulated PBL.
- Research Organization:
- Colorado State Univ., Fort Collins (USA)
- OSTI ID:
- 6859756
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATTLE
CELL KILLING
CELL PROLIFERATION
CHEMICAL COMPOSITION
CHROMIUM 51
CHROMIUM ISOTOPES
CONCANAVALIN
CONNECTIVE TISSUE CELLS
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
GROWTH FACTORS
HEMAGGLUTININS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LECTINS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICROORGANISMS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
RUMINANTS
SHEEP
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATTLE
CELL KILLING
CELL PROLIFERATION
CHEMICAL COMPOSITION
CHROMIUM 51
CHROMIUM ISOTOPES
CONCANAVALIN
CONNECTIVE TISSUE CELLS
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
GROWTH FACTORS
HEMAGGLUTININS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LECTINS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICROORGANISMS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
RUMINANTS
SHEEP
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES