Human gamma interferon production by cytotoxic T lymphocytes sensitized during hepatitis A virus infection

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · J. Virol.; (United States)

OSTI ID:6244255

Maier, K; Gabriel, P; Koscielniak, E; Stierhof, Y D; Wiedmann, K H; Flehmig, B; Vallbracht, A

The production of interferon (IFN) during a chromium-51 release assay with hepatitis A virus (HAV)-infected fibroblasts and autologous peripheral blood lymphocytes from patients with acute HAV infection was studied to determine whether IFN plays a role in immunopathogenesis of hepatitis A infection in humans. Skin fibroblasts of eight patients after acute HAV infection and from two control persons without history of current of past HAV infection were infected with HAV. Peripheral blood lymphocytes were collected at different times after the onset of icterus and tested in a chromium-51 release assay against autologous HAV-infected skin fibroblasts for their cytolytic and IFN-producing activity. The IFN produced during the assay was characterized and found to have the properties of human gamma IFN. Cytotoxicity and gamma IFN release were virus specific. The cell types responsible for both functions were characterized and found to be in the HLA-dependent T8/sup +/ lymphocyte subset. Considering that gamma IFN has an antiviral effect on persistent HAV infection in vitro and that it probably accounts for stimulation of HLA class I antigen expression on hepatocytes, these experimental results presented here demonstrate that human gamma IFN produced by HAV-specific T cells may participate in pathogenesis of hepatitis A infection in humans.

• Research Organization: Univ. of Tuebingen (Germany, F.R.)
• OSTI ID: 6244255
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:10; ISSN JOVIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL KILLING
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FIBROBLASTS
GROWTH FACTORS
IMMUNOLOGY
INFECTIOUS DISEASES
INFECTIOUS HEPATITIS
INTERFERON
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MITOGENS
MONOCLONAL ANTIBODIES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATIENTS
PROTEINS
RADIOASSAY
RADIOISOTOPES
SKIN
SOMATIC CELLS
SYNTHESIS
TOXICITY
VIRAL DISEASES