Cytotoxicity of lymphocytes from melanoma patients against autologous tumor cells and its potentiation in vitro

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

DOI:https://doi.org/10.1007/BF00840152· OSTI ID:5625353

Bykovskaya, S N; Iobadze, M S; Kupriyanova, T A; Demidov, L V

The specific and natural cytotoxicity of peripheral blood lymphocytes from patients with melanomas was compared and stimulation with autologous tumor cells or a pool of allogeneic lymphocytes from five healthy blood donors also was used to potentiate the specific antitumor activity of the patients' lymphocytes. To assess cytolytic ability, cells of an autologous tumor, cells of the K-562 line, autologous peripheral blood lymphocytes, and blast cells obtained from these lymphocytes after stimulation by phytohemagglutinin were used as the target cells. The target cells were incubated in a medium containing sodium chromate and were labelled with the chromium 51 isotope.

• Research Organization: All-Union Oncologic Scientific Center, Moscow, USSR
• OSTI ID: 5625353
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 103:1; ISSN BEXBA
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
550901* -- Pathology-- Tracer Techniques
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANTIBODIES
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CULTURES
CELL KILLING
CHEMISTRY
CHROMATES
CHROMIUM 51
CHROMIUM COMPOUNDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEMAGGLUTININS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYSIS
MATERIALS
MELANOMAS
MITOGENS
MUCOPROTEINS
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PATIENTS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
SODIUM COMPOUNDS
SOMATIC CELLS
TOXICITY
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
TUMOR CELLS