Recovery of immune functions in dogs after total body irradiation and transplantation of autologous blood or bone marrow cells

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Exp. Hematol.; (United States)

OSTI ID:6168777

Pruemmer, O R; Raghavachar, A; Fliedner, T M

The restoration of immune functions was followed in dogs for 101 days after fractionated total body irradiation and autologous transfusion of peripheral blood leukocytes (PBL) or bone marrow (BM) cells. Median numbers of 0.9 X 10(5) granulocyte-macrophage progenitor cells per kilogram of body weight were transferred in either group of recipients. The following parameters recovered more rapidly in PBL recipients as opposed to BM recipients: total blood lymphocyte, T- and B-cell counts, serum levels of immunoglobulins IgM and IgA, in vitro blastogenic responses after stimulation with concanavalin A and pokeweed mitogen, and in vitro plasma cell formation after polyclonal B-cell activation with pokeweed mitogen with or without lipopolysaccharide. No major differences were noted for the restoration of serum IgG levels. Circulating lymphocyte and T-cell numbers remained subnormal for more than three months in both groups, whereas B-cell numbers and serum levels of IgA continued to be depressed in BM recipients only. Thus, autologous PBL restored immune functions more rapidly than did BM. Transplantation of PBL, alone or in addition to autologous BM, might also shorten the period of immunodeficiency after cytoreduction in a variety of malignancies in man.

• Research Organization: Univ. of Ulm, Germany, F.R.
• OSTI ID: 6168777
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 9; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CARBOHYDRATES
CONCANAVALIN
CONNECTIVE TISSUE CELLS
DOGS
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
GLOBULINS
HEMAGGLUTININS
IMMUNE REACTIONS
IMMUNITY
IMMUNOGLOBULINS
IRRADIATION
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
MAMMALS
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
PLASMA CELLS
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RECOVERY
SACCHARIDES
SOMATIC CELLS
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION