Effect of controlled ozone exposure on human lymphocyte function
The effects of ozone (O/sub 3/) on cell-mediated immunity were studied in 16 human subjects exposed to 1176 ..mu..g/m/sup 3/ O/sub 3/ (0.6 ppM) for 2 h in an environmentally controlled exposure chamber. Venous blood samples were taken before and immediately after controlled air and O/sub 3/ exposures, as well as at 72 h, 2 and 4 weeks, and at one random time at least 1 month after treatment. The relative frequency of T lymphocytes in blood and the in vitro blastogenic response of lymphocytes to phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and Candida albicans were determined. During the course of the experiment, no statistically significant changes were observed in the number of T lymphocytes that form spontaneous rosettes with sheep erythrocytes. The response of T lymphocytes to PHA was significantly reduced (P < 0.05) in samples taken at 2 and 4 weeks, following O/sub 3/ exposure. Normal response to PHA was observed at 2 months post-O/sub 3/ exposure. No statistically significant changes in lymphocyte responses to Con A, PWM, or Candida were seen. These results show that one 2 h exposure of humans to 0.6 ppM O/sub 3/ may lead to a transient suppression of the PHA-stimulated blastogenic transformation of peripheral blood lymphocytes. The data indicate that the blastogenic response to PHA of human lymphocytes is exquisitely sensitive to O/sub 3/ exposure and could serve as a bioassay for evaluating subtle changes in cellular immunity induced by O/sub 3/ and possibly other pollutants.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC
- OSTI ID:
- 6453890
- Journal Information:
- Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 24:2; ISSN ENVRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
99 GENERAL AND MISCELLANEOUS
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COUNT
BODY FLUIDS
CARBOHYDRATES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
EXPOSURE CHAMBERS
HEMAGGLUTININS
IMMUNITY
IN VITRO
INHALATION
INTAKE
LEUKOCYTES
LYMPHOCYTES
MATERIALS
METABOLISM
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
OZONE
PATIENTS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
PUBLIC HEALTH
SACCHARIDES
SENSITIVITY
SOMATIC CELLS
TIME DEPENDENCE