Effect of controlled ozone exposure on human lymphocyte function

Journal Article · Wed Apr 01 00:00:00 EST 1981 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(81)90159-6· OSTI ID:6453890

Peterson, M L; Smialowicz, R; Harder, S; Ketcham, B; House, D

The effects of ozone (O/sub 3/) on cell-mediated immunity were studied in 16 human subjects exposed to 1176 ..mu..g/m/sup 3/ O/sub 3/ (0.6 ppM) for 2 h in an environmentally controlled exposure chamber. Venous blood samples were taken before and immediately after controlled air and O/sub 3/ exposures, as well as at 72 h, 2 and 4 weeks, and at one random time at least 1 month after treatment. The relative frequency of T lymphocytes in blood and the in vitro blastogenic response of lymphocytes to phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and Candida albicans were determined. During the course of the experiment, no statistically significant changes were observed in the number of T lymphocytes that form spontaneous rosettes with sheep erythrocytes. The response of T lymphocytes to PHA was significantly reduced (P < 0.05) in samples taken at 2 and 4 weeks, following O/sub 3/ exposure. Normal response to PHA was observed at 2 months post-O/sub 3/ exposure. No statistically significant changes in lymphocyte responses to Con A, PWM, or Candida were seen. These results show that one 2 h exposure of humans to 0.6 ppM O/sub 3/ may lead to a transient suppression of the PHA-stimulated blastogenic transformation of peripheral blood lymphocytes. The data indicate that the blastogenic response to PHA of human lymphocytes is exquisitely sensitive to O/sub 3/ exposure and could serve as a bioassay for evaluating subtle changes in cellular immunity induced by O/sub 3/ and possibly other pollutants.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC
• OSTI ID: 6453890
• Journal Information: Environ. Res.; (United States), Vol. 24:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
IMMUNITY
SENSITIVITY
LYMPHOCYTES
OZONE
BIOLOGICAL EFFECTS
BLOOD COUNT
COMPARATIVE EVALUATIONS
EXPOSURE CHAMBERS
IN VITRO
INHALATION
METABOLISM
PATIENTS
PHYTOHEMAGGLUTININ
PUBLIC HEALTH
TIME DEPENDENCE
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
HEMAGGLUTININS
INTAKE
LEUKOCYTES
MATERIALS
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOMATIC CELLS
560301* - Chemicals Metabolism &amp; Toxicology- Cells- (-1987)
552000 - Public Health
551000 - Physiological Systems
560306 - Chemicals Metabolism &amp; Toxicology- Man- (-1987)
500200 - Environment
Atmospheric- Chemicals Monitoring &amp; Transport- (-1989)