Role of donor passenger leukocytes in rat skin allograft rejection

Journal Article · Sat Dec 01 04:00:00 EST 1973 · Transplantation, v. 16, no. 6, pp. 537-543

OSTI ID:4365175

Kyger, III, E R; Salyer, K E

There has been much speculation on the role of donor passenger leukocytes carried with allografts in the ultimate rejection of these allografts. Results of skin graft donor pretreatment with irradiation, cyclophosphamide, and antilymphocyte serum in various doses and time intervals are presented. Donor pretreatment with irradiation is shown to be superior in prolonging mean graft survival time, and lower doses followed by a longer time interval are shown to be superior to higher doses and a shorter time interval. Much of the difficulty in evaluating previous work on the role of donor passenger leukocytes is attributable to the fact that the exact extent of the donors' hematopoietic chimerism has not been determined. Results are presented using skin grafts from donors that within the limits of bone marrow karyotype analysis and fluorescent antibody study of lymph node sus-pensions are 100% hematopoietic chimeras. Graft from these donors will not sensitize recipients if removed after 48 hr, but will if left in place for 7 days. Untreated control grafts and irradiated isogeneic repopulated grafts sensitize recipients if left in place 48 hr. From these data, the role of donor passenger leukocytes in rat skin allograft rejection would appear to be limited in extent, probably to the period before vascular and lymphatic connections are established between the graft and its host. (auth)

• Research Organization: Univ. of Pennsylvania, Philadelphia
• NSA Number: NSA-29-008026
• OSTI ID: 4365175
• Journal Information: Transplantation, v. 16, no. 6, pp. 537-543, Journal Name: Transplantation, v. 16, no. 6, pp. 537-543; ISSN TRPLA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*GRAFTS-- BIOLOGICAL RADIATION EFFECTS
*IMMUNOSUPPRESSION-- RADIOINDUCTION
GAMMA RADIATION
LEUKOCYTES
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
PHOTON BEAMS
RADIATION CHIMERAS
RATS
SKIN
WHOLE-BODY IRRADIATION
X RADIATION RADIOINDUCTION