THE BEHAVIOR OF THE RADIATION PROTECTIVE AGENT AET IN AQUEOUS BUFFERED SOLUTIONS AND HUMAN SERUM

Technical Report · Wed Aug 28 04:00:00 EDT 1957

OSTI ID:4347925

Shapiro, B

A technic has been developed, utilizing S-35 labeled S,2- aminoethylisothiouronum bromide hydrobromide (AET) and paper chromatography, for identifying the form in which the protective agent exists in aqueous solutions. The products of AET dissolved in solutions of varied pH, temperature, and age were AET, a compound at Rf 0.48, a sulfhydryl compound, and disulfide. The concentrations of these compounds varied, depending on the conditions in the solution. Iradiation of the sulfhydryl form produced disulfide, and irradiation of the disulfide produced additional undentified products. Cystalline AET added to serum yielded the three other products observed in buffered solutions and a protein-bound compound. When the sulfhydryl and disulfide forms were added to serum, only the disulfide and a protein-bound compound appeared. It is suggestod that the compound adminstered may not be the form which protects and it is necessary to know the latter in order to discover the mechinism of protection. (auth)

• Research Organization: Albert Einstein Medical Center. Radiology Dept., Philadelphia
• NSA Number: NSA-12-009009
• OSTI ID: 4347925
• Report Number(s): AF-SAM-58-21
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACIDITY
AET
AGE
BLOOD SERUM
BUFFERS
CHEMISTRY
CHROMATOGRAPHY
CRYSTALS
MAN
ORGANIC SULFUR COMPOUNDS
PAPER
PROTEINS
QUANTITY RATIO
RADIATION EFFECTS
RADIATION PROTECTION
SOLUTIONS
SULFHYDRYL RADICALS
SULFUR 35
SULFUR COMPOUNDS
TEMPERATURE
TRACER TECHNIQUES
WATER