THE BEHAVIOR OF THE RADIATION PROTECTIVE AGENT AET IN HUMAN SERUM IN VITRO

Technical Report · Wed Mar 19 23:00:00 EST 1958

OSTI ID:4312279

Shapiro, B

The behavior of 72-aminoethylisothiuronium bromide hydrobromide (AET) in human sernm was studied by utilizing paper chromatography, paper electrophoresis, and S/sup 35/-labeled AET of high specific activity. On addition of crystalline S/sup 35/ AET to sernm, mercaptoethylguanidine (MEG), guanidinoethyl disulfide, and 2-aminothiazoline (2-AT) are formed and a large portion of radioactivity is bound to the protein. These forms are highly stable and do not change on standing or with irradiation, except in extraordinarily large doses. Addition to human serum of AET in buffered solution containing only MEG and guanidinoethyl disulfide gives the same results as with crystalline AET except that no 2-AT is found. These studies may indicate failure of the radical capture process by products of AET in sernm. However, these results do not reveal the in vivo situation which may be completely different. (auth)

• Research Organization: School of Aviation Medicine, Randolph AFB, Tex.
• NSA Number: NSA-12-013682
• OSTI ID: 4312279
• Report Number(s): AF-SAM-58-91
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACTIVATION ANALYSIS
AET
ANTIMONY
ARSENIC
BIOLOGY AND MEDICINE
BISMUTH
BLOOD SERUM
CHEMICAL REACTIONS
CHROMATOGRAPHY
COPPER
CRYSTALS
ELECTROPHORESIS
FREE RADICALS
GAMMA SPECTROMETERS
IMPURITIES
IRON
LABELLED COMPOUNDS
MAN
MANGANESE
MEG
NEUTRONS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
QUANTITATIVE ANALYSIS
RADIATION DOSES
RADIATION PROTECTION
SILVER
SODIUM
SOLUTIONS
STABILITY
SULFUR 35
VARIATIONS