MECHANISM OF ACTION OF A RADIATION PROTECTIVE AGENT
The radiation protective agent AET (S-(2-aminoethyl) isothiuronium bromide) was labeled with S/sup 35/ and studied in 3 different systems. In the 1st system, AET was irradiated in aqueous buffered solutions, and the radiation products were isolated and identified. In these irradiated solutions, oxidation products of the sulihydryl (MEG) and disulfide (GED) forms of AET, namely, the sulfonic aclds and sulfate, were observed. The 2nd system involved the study of the interaction of MEG and GED wlth protelns. Protein-binding of these protective agents was noted ln a series of protein solutlons, the major bond between the protein and MEG being the mixed disulfide bond. Irradiation of protein solutions containing MEG and GED increased the proteln-binding of these agents. The 3rd system studied was the distribution and metabolism of AET in mice in vlvo. The major form of AET in the mouse was observed to be the protein- bound form. This form correlated best with conditions resulting in protection of the animal agalnst radiation death. (H.H.D.)
- Research Organization:
- Albert Einstein Medical Center, Philadelphia
- NSA Number:
- NSA-16-032851
- OSTI ID:
- 4768491
- Journal Information:
- J. Albert Einstein Med. Center, Journal Name: J. Albert Einstein Med. Center Vol. Vol: 10
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
THE DISTRIBUTION AND METABOLISM OF ORALLY ADMINISTERED 2- MERCAPTOETHYLGUANIDINE (MEG) AND BIS(2-GUANIDOETHYL) DISULFIDE (GED) IN PROTECTED MICE
THE DISTRIBUTION AND THE CHEMICAL FORMS OF THE RADIATION-PROTECTIVE AGENT AET IN MAMMARY TUMOR-BEARING MICE
Related Subjects
BIOLOGY AND MEDICINE
CHEMICAL REACTIONS
DECOMPOSITION
DISTRIBUTION
IRRADIATION
LABELLED COMPOUNDS
METABOLISM
MICE
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
PROTEINS
QUALITATIVE ANALYSIS
RADIATION EFFECTS
RADIATION PROTECTION
SOLUTIONS
SULFATES
SULFIDES
SULFONIC ACIDS
SULFUR 35
WATER