THE DISTRIBUTION AND METABOLISM OF ORALLY ADMINISTERED 2- MERCAPTOETHYLGUANIDINE (MEG) AND BIS(2-GUANIDOETHYL) DISULFIDE (GED) IN PROTECTED MICE
Distribution and metabolism of orally administered 2- mercaptoethylguanidine (MEG) and bis(2-guanidoethyl) disulfide (GED) were observed in order to provide insight into the raechanisra of AET. Male mice fasted overnight were fed /sup 35/S-labeled MEG or GED by cannula. The animals were sacrificed 30 min after feeding and homogenates were prepared for /sup 35/S analysis by liquid scintillation counting. Paper chromatograras and electrophonetograms were scanned for radioactive peaks of metabolites which were measured by planimetry. Identification of metabolites was carried out by synthesis of compounds suspected and comparison of behavior with the unknowns in several chromatographic and electrophoretic systems. Results indicate that MEG is absorbed from the intestinal tract more rapidly than GED. This explains the observation that mice given an oral dose of 400 g/kg MEG 30 min prior to irradiation are better protected than mice given an oral dose of 400 mg/kg GED at the same time. The chemical forms which correlate with protection are P.B.-S/sup AET/, GED, and S-acetyl-2-mercaptoethylguanidine, all of which are higher in the tissues after MEG feeding than after GED feeding. Although the experiments did not reveal the mechanism of action of AET, they do indicate the important chemical forms present in the tissues at the time of radiation, and they suggest manipulations that may improve protection by means of this agent. (H.M.G.)
- Research Organization:
- Albert Einstein Medical Center, Philadelphia
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-18-019772
- OSTI ID:
- 4016676
- Journal Information:
- Annals of the New York Academy of Sciences (U.S.), Journal Name: Annals of the New York Academy of Sciences (U.S.) Vol. Vol: 114; ISSN ANYAA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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FURTHER STUDIES ON THE DISTRIBUTION AND METABOLISM OF 2- MERCAPTOETHYLGUANIDINE AND BIS(2-GUANIDOETHYL) DISULFIDE IN MICE
THE MECHANISM OF ACTION OF AET. II. THE INTERACTION BETWEEN PROTEINS AND 2- MERCAPTOETHYLGUANIDINE AND BIS(2-GUANIDOETHYL) DISULFIDE IN AQUEOUS BUFFERED SOLUTIONS
Related Subjects
BIOLOGY AND MEDICINE
CHROMATOGRAPHY
DETECTION
DIET
DISTRIBUTION
ELECTROPHORESIS
ETHYL RADICALS
GUANIDINE
INTESTINE
LABELLED COMPOUNDS
MEASURED VALUES
MEG
METABOLISM
MICE
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PERFORMANCE
QUANTITATIVE ANALYSIS
RADIATION PROTECTION
RADIOACTIVITY
SAMPLING
SCINTILLATION COUNTERS
STOMACH
SULFIDES
SULFUR 35
TESTING
TISSUES
TRACER TECHNIQUES
VARIATIONS