THE DISTRIBUTION AND THE CHEMICAL FORMS OF THE RADIATION-PROTECTIVE AGENT AET IN LEUKEMIC MICE
The distribution and the chemical nature of the protective agent in the various tissues of C57BL/6J male tumor-bearing mice given a protective dose of 2mercaptoethylguanidine (MEG), the transguanylated form of the radiation- protective agent (AET), S-(2aminoethyl)thiuronium bromide hydrobromide were studied. The mice carried a subcutaneously transplanted leukemia. Results were obtained 20 minutes after an intraperitoneal injection of 140 mg/kg MEG-S/sup 35/ and at 30 minutes after oral administration of 400 mg/kg MEG-S/sup 35/. Under both conditions the tumor took up less protective agent than did most of the vital organs. The tumor contained the same S/sup 35/-labeled chemical forms that normal tissues contained, namely, protein-bound S/sup AET/, GED, 2guanidoethane sulfinic acid, taurocyamine, sulfate, and S-acetyl-2-merc aptoethylguanidine, The protein-bound form consisted mainly of thioester-bound and mixed disulfide-bound protective agent. The tumor had less thioester -bound protective agent than did the small intestines, liver, and kidneys. In vitro studies of protective agent uptake by tissue slices showed that tumor slices took up as much protective agent as did liver or spleen slices. Compared with normal animals, tumor-bearing animals had difficulty excreting the protective agent, apparently as the result of some renal defect. (auth)
- Research Organization:
- Albert Einstein Medical Center, Philadelphia
- NSA Number:
- NSA-18-008202
- OSTI ID:
- 4099849
- Journal Information:
- Cancer Research (U.S.), Journal Name: Cancer Research (U.S.) Vol. Vol: 24; ISSN CNREA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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