EXPERIMENTAL METHODS FOR QUANTITATIVE AUTORADIOGRAPHY IN MICROBIOLOGY AND CELL RESEARCH (in German)
Surface-prepared covered glasses are especially suitable for the production of autoradiographic microbe preparations on account of the definite improvement they offer in regard to microscopic evaluation. The microorganisms are not spread on the slide as ia generally done, but rather are sprayed on the glass with a spraying device and covered with a removable film or liquid emulsion. The pouring of the emulsion is made much easier by the use of a special pouring device. For standardizing the emulsion, activity determinations must be carried out on cell collectives with the usual counter methods, which can be made sufficiently accurate by using an improved pipette technique. The microscopic evaluation of the autoradiographs is carried out by means of silver-grain determination based on a cross-counting procedure, the accuracy of which is discussed. An example of standardization, i.e. the determination of the relationship between the marking of the individual cell and the autoradiographic effect, shows the usefulness of this methcd for quantitative purposes. (auth)
- Research Organization:
- Institut fur Mikrobiologie und Experimentelle Therapie, Jena, Germany
- NSA Number:
- NSA-12-016159
- OSTI ID:
- 4308799
- Journal Information:
- Atompraxis (West Germany) Incorporated in Kerntechnik, Journal Name: Atompraxis (West Germany) Incorporated in Kerntechnik Vol. Vol: 4; ISSN ATPXA
- Country of Publication:
- Country unknown/Code not available
- Language:
- German
Similar Records
Direct Approach to Quantitative Autoradiography
A METHOD OF ELECTRON MICROSCOPIC AUTORADIOGRAPHY WITH TRITIUM-LABELED CELLS IN A MAGNETIC FIELD