A METHOD OF ELECTRON MICROSCOPIC AUTORADIOGRAPHY WITH TRITIUM-LABELED CELLS IN A MAGNETIC FIELD

Journal Article · Mon May 01 04:00:00 EDT 1961 · Laboratory Investigation (U.S.)

OSTI ID:4738952

Harford, C G; Hamlin, A

BS>A method is described that permits electron microscopy of autoradiographs obtained with H/sup 3/-labeled biological materials. Tracks produced in film emulsion by emitted beta particles were directiy observed as reacted Ag grains formed by reduction of AgBr. Thin sections of HeLa cells labeled with tritiated thymidine were mounted on grids, covered with nuclear track emulsion, incubated, developed, and examined in the electron microscope. Reacted grains of the emulsion were found to have a distinctive appearance in the electron microscope because of a filamentous shape, and the identity of such grains was established by suitable controls. Because of distinct nuclear membranes in the electron micrographs, the location of reacted grains could be determined easily, and, although most of the grains were intranuclear, some scatter persisted. When the grids were exposed to a magnetic field during incubation, scatter was also not completely eliminated but a marked increase in number of reacted grains was found in the nuclei of the cells. The procedure appears to have advantages over previously used autoradiographic methods for intracelluiar locaiization of H/sup 3/-labeled compounds. (H.H.D.)

• Research Organization: Washington Univ., St. Louis
• NSA Number: NSA-17-010284
• OSTI ID: 4738952
• Journal Information: Laboratory Investigation (U.S.), Journal Name: Laboratory Investigation (U.S.) Vol. Vol: 10; ISSN LAINA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BETA PARTICLES
BIOLOGY AND MEDICINE
ELECTRON MICROSCOPY
NUCLEAR EMULSIONS
PARTICLE TRACKS
RADIOAUTOGRAPHY
SILVER BROMIDES
TRACER TECHNIQUES
TRITIUM