Differential Autoradiography of Tritium and Another β-Emitter by a Double Stripping Film Technique
An autoradiographic technique for studying the incorporation of two metabolites or the same metabolite at different times by a group of cells, was demonstrated with HeLa cells exposed flrst to tritiated thymidine and then to C14-labeled thymidine. The specific activities were adjusted so that the exposure required for autoradiography of the tritiated compound was ~1/10 of that required for C14. After fixing and staining, the cells were coated with autoradiographic emulsions by the stripping-film technique, the emulsion being without a gelatin supporting layer and thick enough to prevent penetration of tritium BETA particles. After a 1-day exposure to record the tritium, the emulsion was processed to give blue dye-coupled grains. The first autoradiograph was next coated with a second stripping emulsion and processed after 10 days' exposure to give black grains for Cl4. The two images could be separated in the microscope by differential focusing.
- Research Organization:
- Royal Cancer Hospital, London
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-026707
- OSTI ID:
- 4797070
- Journal Information:
- Nature (London), Journal Name: Nature (London) Journal Issue: 4840 Vol. 195; ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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