Evaluation of section thickness of skin in determining the epidermal labeling index with tritiated thymidine

Journal Article · Mon Jan 01 04:00:00 EST 1973 · Arch. Dermatol. Forsch., v. 247, pp. 207-210

DOI:https://doi.org/10.1007/BF00596239· OSTI ID:4308767

Taguchi, Y H; Tabachnick, J

Epidermal basal cells in the albino guinea pig were pulse labeled with tritiated thymidine (/sup 3/H-TdR) by intraperitoneal or intradermal injection, and the fraction of labeled cells STAlabeling index (LI)! was compared in 4 mu versus 6 mu sections. The LI scored in 6 mu sections was about 10% lower than that in 4 mu sections. For our purpose, it is unnecessary to submit to the technical difficulties of routinely cutting sections of skin thinner than 5 to 6 mu for autoradiography of /sup 3/H-TdR, since the increase in scoring efficiency is relatively small (10 to 20%). (auth)

• Research Organization: Albert Einstein Medical Center, Philadelphia
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-027173
• OSTI ID: 4308767
• Journal Information: Arch. Dermatol. Forsch., v. 247, pp. 207-210, Journal Name: Arch. Dermatol. Forsch., v. 247, pp. 207-210; ISSN ADMFA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*EPIDERMIS-- LABELLING
*SKIN-- THICKNESS
AUTORADIOGRAPHY
GUINEA PIGS
N48830* --Life Sciences--New Tracer Techniques--Other
PULSE TECHNIQUES
THYMIDINE
TRACER TECHNIQUES
TRITIUM