Effect of pituitary gonadotrophins on tritiated thymidine uptake by rat ovary. An autoradiographic study

Journal Article · Tue Dec 31 23:00:00 EST 1974 · Arch. Gynaekol., v. 218, no. 1, pp. 1-9

DOI:https://doi.org/10.1007/BF00672278· OSTI ID:4124258

Nakano, R; Mizuno, T; Katayama, K; Hayashi, K; Tojo, S

The effect of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on the follicular growth in the ovary of the hypophysectomized rat was investigated using autoradiography. The numbers of DNA-synthesizing nuclei in the granulosa cell were measured by autoradiography after flashlabelling with tritiated ($sup 3$H) thymidine. The frequency of $sup 3$H-thymidine labelled nuclei in the granulosa cell enhanced in the presence of FSH. In contrast, LH had no significant effect on thymidine uptake. The result suggests that FSH stimulates follicle cell division, whereas LH does not. (orig.)

• Research Organization: Kobe Univ. (Japan). Dept. of Obstetrics and Gynecology
• NSA Number: NSA-33-009687
• OSTI ID: 4124258
• Journal Information: Arch. Gynaekol., v. 218, no. 1, pp. 1-9, Journal Name: Arch. Gynaekol., v. 218, no. 1, pp. 1-9; ISSN ARGYA
• Country of Publication: Germany
• Language: English

Related Subjects

*DNA-- BIOSYNTHESIS
*FSH-- BIOLOGICAL EFFECTS
*LH-- BIOLOGICAL EFFECTS
*OVARIES-- AUTORADIOGRAPHY
551001* --Biomedical Sciences
Basic Studies--Physiological Systems--Tracer Techniques
CELL DIVISION
HYPOPHYSECTOMY
N48830* --Life Sciences--New Tracer Techniques--Other
PITUITARY GLAND
RATS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE