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Metabolism of testosterone by human granulosa cells in culture: influence of follicle-stimulating hormone and luteinizing hormone

Journal Article · · Am. J. Obstet. Gynecol.; (United States)
OSTI ID:5242422
; ; ;

Human granulosa cells were isolated from follicles (8 to 15 mm) and cultivated for 24 hours in the presence or absence of follicle-stimulating hormone (NIH-FSH-HS-1, 1 microgram/ml) and luteinizing hormone (NIAMDD-hLH-1, 1 microgram/ml). Testosterone -4-14C was added subsequently to all cultures for 4-, 6-, and 24-hour periods. Of the seven metabolites of testosterone studied, 17 beta-estradiol (E2) and estrone (E1) were the major products. In all patients, levels of E2 were three to ten times higher than those of E1. Production of E2, but not E1, was stimulated by either follicle-stimulating hormone (FSH) or luteinizing hormone (LH). The cells of the largest follicle (15 mm) showed greater response to LH than to FSH. Production of the other C19 and C18 metabolites was very low or negligible. These results further suggest that FSH regulates the aromatization of testosterone in human granulosa cells, and that LH may have the same effect on the matured follicle during the preovulatory period.

Research Organization:
Department of Obstetrics and Gynaecology, Vancouver General Hospital, University of British Columbia, Canada
OSTI ID:
5242422
Journal Information:
Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Vol. 142:6; ISSN AJOGA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADULTS
AGE GROUPS
ANDROGENS
ANDROSTANES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CELL CULTURES
ESTRADIOL
ESTRANES
ESTROGENS
ESTRONE
FEMALE GENITALS
FEMALES
FSH
GONADOTROPINS
GONADS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
LH
MAMMALS
MAN
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
ORGANS
OVARIES
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
STEROID HORMONES
STEROIDS
TESTOSTERONE
VERTEBRATES
WOMEN