Differential responsiveness of luteinized human granulosa cells to gonadotropins and insulin-like growth factor I for induction of aromatase activity

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Fertility and Sterility; (United States)

OSTI ID:5398772

Christman, G M; Randolph, Jr, J F; Peegel, H; Menon, K M ^[1]

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1. Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor (USA)

The objective of this study was to examine the in vitro responsiveness of cultured luteinized human granulosa cells over time to insulin-like growth factor 1 (IGF-1), human follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) for the induction of aromatase activity. Granulosa cells were retrieved from preovulatory follicles in patients undergoing in vitro fertilization. Cells were cultured for a period of 72 hours or 10 days. The ability of hCG, human FSH, and/or IGF-I to induce aromatase activity was assayed by the stereospecific release of tritium from (1B-3H)androstenedione. Short-term cultures (72 hours) demonstrated a marked rise in aromatase activity in response to human FSH and IGF-I, whereas a smaller response to hCG was observed. In contrast, 10-day cultures demonstrated responsiveness predominantly to hCG rather than human FSH for the induction of aromatase activity with no remarkable effect of IGF-I. Luteinized human granulosa cells undergo a transformation from an initial human FSH and IGF-I responsive state to an hCG responsive state in long-term cultures.

• OSTI ID: 5398772
• Journal Information: Fertility and Sterility; (United States), Journal Name: Fertility and Sterility; (United States) Vol. 55:6; ISSN FESTA; ISSN 0015-0282
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
FSH
GENE REGULATION
GONADOTROPINS
GROWTH FACTORS
HORMONES
HYDROGEN COMPOUNDS
IN VITRO
ISOMERASES
ISOTOPE APPLICATIONS
KINETICS
MITOGENS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS