PREPARATION OF TRITIUM-LABELED THYMIDINE AND ITS USE FOR THE STUDY, BY THE RADIOAUTOGRAPHIC METHOD, OF THE SYNTHESIS OF DEOXYRIBONUCLEIC ACID IN CELLS BEING CULTURED
The biosynthesis of desoxyribonucleic acid was studied in chicken fibroblasts. Autoradiograms were made of tissues following culture in a medium containing tritium-labeled thymidine. Incorporation of labeled thymidine in the nucleus precedes mitosis by about 7 hours and coincides statistically with the increase in quantity of desoxyribonucleic acid in the nucleus as measured by Feulgen's test. At the time of division, the newly formed desoxyribonucleic acid is distributed equally between the two sets of chromosomes that are to form the nuclei of the daughter cells. After three days of contact with tritium-labeled thymidine, there remain some nuclei that are not labeled. These nuclei do not seem to participate in the mitotic cycles; the technique employed shows no synthesis of desoxyribonucleic acid. There is no turnover of such nucleic acid, nor exchange of its thymidine fraction. (C.H.)
- Research Organization:
- Univ. of Liege
- NSA Number:
- NSA-13-006111
- OSTI ID:
- 4293600
- Report Number(s):
- A/CONF.15/P/323
- Resource Relation:
- Other Information: Orig. Receipt Date: 31-DEC-59
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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