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Title: Incorporation of Tritium-labelled Thymidine and Tritium-labelled Deoxycytidylic Acid into Deoxyribonucleic Acid after Irradiation of the Deoxyribonucleic Acid Primer with γ-Rays

Abstract

Results are reported from a study of the ability of irradiated and non- irradiated desoxyribonucleic acid to incorporate tritium-labeled thymidine or tritium-labeled desoxycytidylic acid in the presence of the desoxyribonucleic acid-synthesizing enzyme system from regenerating rat liver. Desoxyribonucleic acid isolated from call thymus was used in all experiments. An aqueous solution of the acid was irradiated with various doses of gamma radiation. Irradiated, as well as non-irradiated, samples were used as primers to study the incorporation of either labeled thymidine or labeled desoxycytidylic acid into desoxyribonucleic acid in the presence of desoxyadenylic acid, desoxyguanylic acid, desoxycytidylic acid, thymidine, adenosine triphosphate, and the desoxyribonucleic acidsynthesizing enzyme system prepared from regenerating rat liver. Results are tabulated.

Authors:
Publication Date:
Research Org.:
Univ. of Rochester, N.Y.
Sponsoring Org.:
USDOE
OSTI Identifier:
4188348
NSA Number:
NSA-14-007206
Resource Type:
Journal Article
Journal Name:
Nature (London)
Additional Journal Information:
Journal Volume: 185; Journal Issue: 4707; Other Information: Orig. Receipt Date: 31-DEC-60; Journal ID: ISSN 0028-0836
Publisher:
Nature Publishing Group
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; ATP; BIOCHEMISTRY; CYTIDYLIC ACID; DNA; ENZYMES; GAMMA RADIATION; LABELLED COMPOUNDS; LIVER; NUCLEIC ACIDS; NUCLEOTIDES; ORGANIC ACIDS; PHOSPHATES; RADIATION DOSES; RADIATION EFFECTS; RATS; REGENERATION; SAMPLING; TABLES; THYMIDINE; THYMUS; TRITIUM; VARIATIONS

Citation Formats

OKADA, S. Incorporation of Tritium-labelled Thymidine and Tritium-labelled Deoxycytidylic Acid into Deoxyribonucleic Acid after Irradiation of the Deoxyribonucleic Acid Primer with γ-Rays. Country unknown/Code not available: N. p., 1960. Web. doi:10.1038/185193a0.
OKADA, S. Incorporation of Tritium-labelled Thymidine and Tritium-labelled Deoxycytidylic Acid into Deoxyribonucleic Acid after Irradiation of the Deoxyribonucleic Acid Primer with γ-Rays. Country unknown/Code not available. doi:10.1038/185193a0.
OKADA, S. Sat . "Incorporation of Tritium-labelled Thymidine and Tritium-labelled Deoxycytidylic Acid into Deoxyribonucleic Acid after Irradiation of the Deoxyribonucleic Acid Primer with γ-Rays". Country unknown/Code not available. doi:10.1038/185193a0.
@article{osti_4188348,
title = {Incorporation of Tritium-labelled Thymidine and Tritium-labelled Deoxycytidylic Acid into Deoxyribonucleic Acid after Irradiation of the Deoxyribonucleic Acid Primer with γ-Rays},
author = {OKADA, S.},
abstractNote = {Results are reported from a study of the ability of irradiated and non- irradiated desoxyribonucleic acid to incorporate tritium-labeled thymidine or tritium-labeled desoxycytidylic acid in the presence of the desoxyribonucleic acid-synthesizing enzyme system from regenerating rat liver. Desoxyribonucleic acid isolated from call thymus was used in all experiments. An aqueous solution of the acid was irradiated with various doses of gamma radiation. Irradiated, as well as non-irradiated, samples were used as primers to study the incorporation of either labeled thymidine or labeled desoxycytidylic acid into desoxyribonucleic acid in the presence of desoxyadenylic acid, desoxyguanylic acid, desoxycytidylic acid, thymidine, adenosine triphosphate, and the desoxyribonucleic acidsynthesizing enzyme system prepared from regenerating rat liver. Results are tabulated.},
doi = {10.1038/185193a0},
journal = {Nature (London)},
issn = {0028-0836},
number = 4707,
volume = 185,
place = {Country unknown/Code not available},
year = {1960},
month = {1}
}