Incorporation of Tritium-labelled Thymidine in Bufo $female$ × Rana temporaria $male$ Hybrid Embryos
Two-cell stages of hybrid embryos resulting from the cross-fertilization of Bufo and Rana temporaria were incubated for 17 hrs in a medium containing tritium-labeled thymidine. The embryos were fixed by freeze-substitution and the incorporation of tritium studied by the radioautographic technique. The embryos stopped development at the late blastula stage. Labeling of desoxyribonucleic acid was demonstrated in morula as well as in blastula cells of the lethal hybrids. Tritium-labeled thymidine was shown to be incorporated into desoxyribonucleic acid 24 hr after development stopped, which suggests that the block in development was not due to the arrest of desoxyribonucleic acid synthesis. (C.H.)
- Research Organization:
- Universite Libre, Brussels
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-015367
- OSTI ID:
- 4041105
- Journal Information:
- Nature (London), Journal Name: Nature (London) Journal Issue: 4770 Vol. 190; ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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