THE EFFECT OF SELF- AND EXTERNAL RADIATIONS ON I$sup 131$-LABELLED 1- THYROXINE AND 3,5,3'-TRIIODO-1-THYRONINE IN SOLUTION
Chemical changes produced in dilute solutions of I/sup 131/labeled l- thyroxine and 3,5,3'-triiodo-l-thyronine by the action of self- and external radiations (high energy x and radiation) have been studied quantitatively. The same major products of radiochemical decomposition, Compounds "1" and "2", were obtained from selfradiation in labeled thyroxine and triiodothyronine of high specific activity and from externally irradiated samples of low specific activity. By a combination of chromatographic, electrophoretic and spot-test analyses, Compound "1" has been tentatively identified as gestion for the formation of a similar derivative of triiodothyronine. The kinetics of radiochemical change induced by self-radiation of the two iodothyronines have been studied over a period of storage of 40 days. Once formed, the lactic acid analogues of the hormones are rapidly deiodinated. The radiochemical reaction is inhibited by storage in the dried or frozen state or by the addition of cysteines glycine and human serum albumin. The possible mechanism of the radiationinduced changes and their implications in biological work are discussed. (auth)
- Research Organization:
- National Inst. for Medical Research, London
- NSA Number:
- NSA-13-014162
- OSTI ID:
- 4255046
- Journal Information:
- Clinica Chimica Acta (Netherlands), Vol. Vol: 4; Other Information: Orig. Receipt Date: 31-DEC-59
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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ALBUMINS- AMINO ACIDS- BIOLOGY- BLOOD SERUM- CHEMICAL REACTIONS- CYSTEINE- GLYCINE- HUMIDITY- LOW TEMPERATURE- MAN- RADIATION EFFECTS- RADIOCHEMISTRY- STORAGE- VARIATIONS
CHEMICAL REACTIONS- CHROMATOGRAPHY- COMPOUNDS- DECOMPOSITION- ELECTROPHORESIS- ENERGY- GAMMA RADIATION- HORMONES- IODINE 131- LABELLED COMPOUNDS- LACTIC ACID- ORGANIC ACIDS- QUANTITY RATIO- RADIATION EFFECTS- RADIOACTIVITY- RADIOCHEMISTRY- REACTION KINETICS- SOLUTIONS- STORAGE- THYROID- THYRONINE- THYROXINE- TRACER TECHNIQUES- X RADIATION