Proliferative Potentials of Bone Marrow and Blood Cells Studied by in vitro Uptake of H³-Thymidine

Journal Article · Thu Jan 01 00:00:00 EST 1959 · Acta Haematologica

DOI:https://doi.org/10.1159/000205651· OSTI ID:4234760

Bond, V. P.; Fliedner, T. M.; Cronkite, E. P.; Rubini, J. R.; Brecher, G.; Schork, P. K.

Cell proliferative activity and potential in the circulating blood and in the bone marrow of individuals with normal hematopoiesis, and in patients with hematopoietic dyscrasias was studied by means of in vitro one hour incubation with tritiated thymidine (H³Th) and 6tripping film autoradiography. The labeled material is incorporated only into DNA during synthesis. In normal bone marrow, labeling was seen at 1 hour in all cell lineages, and in cells variously referred to as "reticulum," "stem,'' " stroma,'' etc., cells. Erythropoietic cells were labeled as far as the polychromatic normoblast; the myeloid series was labeled to the myelocyte state. Leukemia cells in the bone marrow and peripheral blood of patients with acute or chronic myelocytic leukemia incorporated label avidly; the small typical leukemia cell of chronic lymphocytic leukemia did not label at all. Less than 3 per cent of the myeloma cells in patients with multiple myeloma incorporated thymidine. Most striking was the finding of small numbers of labeled large mononuclear cells of different morphological types in the peripheral blood of normal human beings, and an increase in the number of morphologically identical cells in the blood of patients with infection and infectious mononucleosis. The labeling indicates active DNA synthesis and thus these cells presumably are capable of division. It is suggested that these cells may represent a mobile pool of primitive progenitor cells and are multipotential in their function.

• Research Organization: Brookhaven National Lab., Upton, N.Y.
• Sponsoring Organization: USDOE
• NSA Number: NSA-13-021838
• OSTI ID: 4234760
• Journal Information: Acta Haematologica, Vol. 21, Issue 1; Other Information: Orig. Receipt Date: 31-DEC-59; ISSN 0001-5792
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
BIOCHEMISTRY
BLOOD CELLS
BLOOD FORMATION
BONE MARROW
CYTOLOGY
DNA
ERYTHROCYTES
FILMS
HETEROCYCLICS
INFECTIONS
LABELLED COMPOUNDS
LEUKEMIA
LYMPHOCYTES
MAN
MITOSIS
NUCLEIC ACIDS
NUCLEOSIDES
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIOAUTOGRAPHY
REGENERATION
THYMIDINE
THYMINE
TRACER TECHNIQUES
TRITIUM