Simultaneous determination of testosterone, 5$alpha$-androstan-17$beta$-ol- 3-one, 5$alpha$-androstane-3$alpha$,17$beta$-diol and 5$alpha$-androstane-3$beta$,17$beta$-diol in plasma of adult male rabbits by radioimmunoassay

Journal Article · Wed Oct 01 00:00:00 EDT 1975 · Endocrinology, v. 97, no. 4, pp. 787-792

OSTI ID:4181679

Schanbacher, B D; Ewing, L L

A simultaneous radioimmunoassay procedure for plasma testosterone (T), 5alpha-androstan-17beta-ol-3-one (DHT), 5alpha- androstane-3alpha,17beta-diol (3alphaol), and 5alpha-androstan- 3beta,17beta-diol (3betaol) is described in this report. Peripheral plasma concentrations of T, DHT, 3alphaol, and 3$beta$ol were 1.61 +- 0.26, 0.49 +- 0.15, 0.17 +- 0.03, and 0.24 +- 0.04 ng/ml, respectively, in adult male rabbits. In contrast, T, DHT, 3$alpha$ol, and 3$beta$ol peripheral plasma concentrations in dexamethasone-treated castrate rabbits were 0.07 +- 0.001, 0.02 +- 0.01, 0.07 +- 0.01, and 0.05 +- 0.01 ng/ml, respectively. These results represent the first simultaneous measurement of T, DHT, 3$alpha$ol, and 3$beta$ol in peripheral plasma of any male vertebrate. Moreover, we suggest that DHT, 3$alpha$ol, and 3$beta$ol represent the unidentified immunoreactive material found in peripheral plasma of male rabbits by Falvo and Nalbandov. (auth)

• Research Organization: Johns Hopkins Univ., Baltimore
• NSA Number: NSA-33-003327
• OSTI ID: 4181679
• Journal Information: Endocrinology, v. 97, no. 4, pp. 787-792, Journal Name: Endocrinology, v. 97, no. 4, pp. 787-792; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

*ANDROSTANES-- RADIOIMMUNOASSAY
*TESTOSTERONE-- RADIOIMMUNOASSAY
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
ACCURACY
BLOOD PLASMA
CASTRATION
DEXAMETHASONE
IMMUNE SERUMS
N48830* --Life Sciences--New Tracer Techniques--Other
RABBITS
SENSITIVITY
TRITIUM COMPOUNDS