Urinary 5. cap alpha. -androstane-3. cap alpha. ,17. beta. -diol radioimmunoassay: a new clinical evaluation. [Tritium tracer technique]
A rapid specific and reliable RIA for urinary 5..cap alpha..-androstane-3..cap alpha.., 17..beta..-diol (Adiol) is described using chromatographical purification and a specific antibody. Values are reported under some physiological and pathological conditions in 179 individuals. In 43 normal adult men the mean (+- SD) urinary Adiol excretion was 193 +- 77 ..mu..g/24 h, and in 29 normal women it was 44 +- 23 ..mu..g/24 h. These values are significantly different (P < 0.01). In 49 hirsute women, urinary Adiol Excretion was elevated (137 +- 51 ..mu..g/24 h) and significantly different from this value in normal women (P < 0.01). The urinary Adiol excretion in 10 postmenopausal women was very low (< 5 ..mu..g/24 h). In normal adult subjects, the theoretical contribution to urinary Adiol of the major secreted androgens was calculated. Whereas dehydroisoandrosterone and dehydroisoandrosterone sulfate yield the same amount of urinary Adiol in both sexes, testosterone is the main precursor of Adiol in men and androstenedione is the main precursor in normal premenopausal and hirsute women. However, the amount of Adiol recovered in the 24-h urine depends not only on the secretion rate of androstenedione and testosterone but is also related to the testosterone 5..cap alpha..-reductase activity present in androgen target cells, especially in sexual skin.
- Research Organization:
- Faculty of Medicine, Paris
- OSTI ID:
- 6540954
- Journal Information:
- J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 47:4; ISSN JCEMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ANDROSTANES
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROIDS
TRACER TECHNIQUES
TRITIUM
URINE
WASTES
YEARS LIVING RADIOISOTOPES