Metabolism of (/sup 3/H) 5 alpha-androstane-3 alpha, 17 beta-diol by cultures of isolated rat sertoli cells and the effect of LH and FSH

Journal Article · Sat Mar 31 23:00:00 EST 1984 · Steroids; (United States)

DOI:https://doi.org/10.1016/0039-128X(84)90019-9· OSTI ID:5655741

Tcholakian, R K; Berkowitz, A S; Newaz, S N

Sertoli cells from immature rats metabolized (/sup 3/H) 5 alpha-androstane-3 alpha, 17 beta-diol to (/sup 3/H) 5 alpha-androstane-3 alpha, 16 alpha, 17 beta-triol and (/sup 3/H) 3 alpha-hydroxy-5 alpha-androstan-17-one. This is the first report of 16 alpha-hydroxylation of 5 alpha-reduced androgens in the testis. FSH significantly stimulated 16 alpha-hydroxylation while LH significantly decreased this activity. 3 alpha-Hydroxy-5 alpha-androstan-17-one was the major metabolite formed and its production was significantly increased in the presence of both LH and FSH, although FSH stimulation was significantly more than LH. The possible role of 16 alpha-hydroxylase in androgen metabolism by immature rat Sertoli cells is discussed.

• Research Organization: Univ. of Texas Medical Schoo, Houston
• OSTI ID: 5655741
• Journal Information: Steroids; (United States), Journal Name: Steroids; (United States) Vol. 43:4; ISSN STEDA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANDROGENS
ANDROSTANES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CELL CULTURES
ENZYMES
FSH
GLYCOLS
GONADOTROPINS
GONADS
HORMONES
HYDROXY COMPOUNDS
HYDROXYLASES
HYDROXYLATION
LH
MALE GENITALS
MAMMALS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PITUITARY HORMONES
RATS
RODENTS
STEROID HORMONES
STEROIDS
TESTES
VERTEBRATES