Metabolism of /sup 3/H-estradiol-17 beta by cultures of isolated rat Sertoli cells and the effect of FSH: presence of 16 alpha-hydroxylase

Journal Article · Fri Jul 01 00:00:00 EDT 1983 · Steroids; (United States)

DOI:https://doi.org/10.1016/S0039-128X(83)90084-3· OSTI ID:6834547

Tcholakian, R K; Steinberger, A; Pyrek, J

The ability of Sertoli cells to metabolize /sup 3/H-estradiol-17 beta was investigated utilizing Sertoli cell cultures isolated from 18d rat testes. The Sertoli cells converted estradiol-17 beta to estriol as shown by recrystallization of estriol from samples containing cells and media but not from cell-free control media. The effect of FSH treatment on such metabolism was investigated and was shown to be similar to nontreated samples. This is the first demonstration that 16 alpha-hydroxylase is present in Sertoli cells and that this enzyme activity is not under the influence of FSH.

• Research Organization: Department of Obstetrics, Gynecology, University of Texas Medical School-Houston
• OSTI ID: 6834547
• Journal Information: Steroids; (United States), Journal Name: Steroids; (United States) Vol. 42:1; ISSN STEDA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
ENZYME ACTIVITY
ENZYMES
ESTRADIOL
ESTRANES
ESTROGENS
FSH
GONADOTROPINS
GONADS
HORMONES
HYDROXY COMPOUNDS
HYDROXYLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PITUITARY HORMONES
RATS
RODENTS
STEROID HORMONES
STEROIDS
TESTES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES