Radioimmunoassay of group I pepsinogens in serum

Journal Article · Mon Apr 01 04:00:00 EDT 1974 · Gastroenterology, v. 66, no. 4, pp. 494-502

OSTI ID:4166853

Samiloff, I M; Liebman, W M

A radioimmunoassay for the group I pepsinogens (PG I) in human serum has been developed. The standard PG I was isolated from human urine, and, when labeled with $sup 125$I, was shown to be immunochemically homogeneous by radioimmunoelectrophoresis and radioimmunoprecipitation. The antiserum was obtained from rabbits immunized with partially purified PG I. Standard PG I and serum give parallel inhibition curves against the labeled antigen. The sensitivity of the assay is in the order of 2 ng per ml of serum. The reproducibility of the determinations was demonstrated. PG I in serum is stable when stored at -20$sup 0$C for at least 7 months. The mean (+-SD) serum PG I levels in 69 healthy control subjects and in 71 hospitalized control patients were 102.7 +- 32.5 and 96.2 +- 41.9 ng per ml, respectively. In 14 patients with pernicious anemia and in 12 patients with total gastrectomy, mean serum PG I levels were 10.2 +- 6.4 and 3.8 +- 2.9 ng per ml, respectively. In 5 patients with Zollinger-Ellison syndrome, the mean level was 456.1 +- 187.3 ng per ml. (auth)

• Research Organization: Harbor General Hospital, Torrance, CA
• NSA Number: NSA-33-005856
• OSTI ID: 4166853
• Journal Information: Gastroenterology, v. 66, no. 4, pp. 494-502, Journal Name: Gastroenterology, v. 66, no. 4, pp. 494-502; ISSN GASTA
• Country of Publication: United States
• Language: English

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