Identification and measurement of a folate-binding protein in human serum by radioimmunoassay

Journal Article · Thu Jun 01 04:00:00 EDT 1978 · J. Lab. Clin. Med.; (United States)

OSTI ID:6845281

Da Costa, M; Rothenberg, S P; Fischer, C; Rosenberg, Z

Antiserum raised in rabbits against the FBP obtained from CML cells, and the purified binder labeled with /sup 125/I, have been used for an RIA which can measure an immunologically similar protein in human serum. The concentration of the binding protein in normal serums ranged from 1.2 to 9.3 ng/ml, with a mean +- S.E.M. of 3.8 +- 0.4 ng/ml. Elevated values of the binder protein were measured in the serums from patients with folate deficiency, vitamin B/sub 12/ deficiency, liver disease, uremia, myeloproliferative diseases, chronic lymphocytic leukemia, and various types of cancer and in the serum from pregnant women. The concentration of the binder protein and the capacity of the serum to specifically bind isotopically labeled PGA correlated poorly, indicating that the binding protein concentration and degree of saturation by endogenous serum folate vary independently in many instances.

• Research Organization: New York Medical Coll., New York
• OSTI ID: 6845281
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 91:6; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MAN
METABOLIC DISEASES
NEOPLASMS
NUCLEI
NUTRITIONAL DEFICIENCY
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PREGNANCY
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES