Pepsinogens I and II: purification from gastric mucosa and radioimmunoassay in serum

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Gastroenterology; (United States)

OSTI ID:5346916

Samloff, I M

Pepsinogen I and pepsinogen II were purified from gastric mucosa and used to develop a radioimmunoassay for pepsinogen II and an improved radioimmunoassay for pepsinogen I. Each immunochemically homogeneous preparation contained only its characteristic components by radioelectophoretic analysis, and migrated as a singly band in polyacrylamide gel. The mean (+/- SD) level of serum pepsinogen II in 42 healthy control subjects was 10,8 +/- 3.8 ng/ml, significantly less (p less than 0.001) than the level of pepsinogen I, which was 62.9 +/- 22.2 ng/ml. The correlation between serum pepsinogen I and pepsinogen II was highly significant (r . 0.700, p less than 0.001) in these subjects. In 20 patients with pernicious anemia the mean serum pepsinogen II level was 10.6 +/- 2.5 ng/ml, not different from normal, but significantly higher (p less than 0.001) than the level of pepsinogen I which was 5.9 +/- 4.7 ng/ml. IN 10 patients with total gastrectomy, serum pepsinogen I was 3.9 +/- 3.1 ng/ml and serum pepsinogen II was 3.2 +/- 3.1 ng/ml; both values were significantly lower (p less than 0.001) than the corresponding levels in pernicious anemia. The predominance of pepsinogen I in the serum of healthy control subjects suggests that either the gastric chief cell normally releases more pepsinogen I than pepsinogen II into the circulation or that pepsinogen I has longer metabolic clearance rate than pepsinogen II. The marked decrease in serum pepsinogen I in patients with pernicious anemia is best explained by a loss of gastric chief cells due to severe atropic gastritis of the fundic glands. The normal distribution of serum pepsinogen II levels in these patients may reflect an increased number of pyloric glands due to pyloric gland metaplasia of the proximal stomach.

• Research Organization: Department of Medicine, Harbor-UCLA Medical Center, Torrance, California
• OSTI ID: 5346916
• Journal Information: Gastroenterology; (United States), Journal Name: Gastroenterology; (United States) Vol. 82:1; ISSN GASTA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID PROTEINASES
ANEMIAS
BLOOD SERUM
BODY
CELL PROLIFERATION
DIGESTIVE SYSTEM
DISEASES
ELECTROPHORESIS
ENZYMES
GASTRECTOMY
GASTROINTESTINAL TRACT
HEMIC DISEASES
HYDROLASES
ISOTOPE APPLICATIONS
MEDICINE
MEMBRANES
MUCOUS MEMBRANES
ORGANS
PATIENTS
PEPSIN
PEPTIDE HYDROLASES
PRECURSOR
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
STOMACH
SURGERY
SYMPTOMS
TRACER TECHNIQUES