A radioimmunoassay for erythropoietin: serum levels in normal human subjects and patients with hemopoietic disorders

Journal Article · Wed Dec 01 04:00:00 EST 1982 · J. Lab. Clin. Med.; (United States)

OSTI ID:6377731

Rege, A B; Brookins, J; Fisher, J W

An RIA for Ep has been developed that is highly sensitive and specific. A homogeneous Ep preparation was labeled with /sup 125/I by the chloramine-T method to a specific activity of 90 to 136 micro Ci/microgram and immunoreactivity of 80%. Ep antiserum, which was produced to a human urinary Ep preparation (80 U/mg of protein), was adsorbed with normal human urinary and serum proteins without any loss in sensitivity of the RIA to increase the specificity of the assay. A good correlation was seen between the RIA and the exhypoxic polycythemic mouse assay (corr. coef. 0.967; slope 1.05 and y intercept 0.75). Ep titers in sera from 175 hematologically normal human subjects exhibited a normal frequency distribution and ranged between 5.8 and 36.6 mU/ml with a mean of 14.9 +/- 4.7 (S.D.) and median of 14.3 Serum Ep titers were markedly elevated in seven patients with aplastic anemia and one patient with pure red cell aplasia (1350 to 20,640 mU/ml) and were lower than normal in two patients with polycythemia vera (8.1 and 9.4 mU/ml). The serum Ep titers in a prenephrectomy patient with chronic glomerulonephritis (32.1 mU/ml) decreased to below normal levels (9.04 mU/ml) after nephrectomy. The cord serum erythropoietin titers in 10 IDM (90.82 +/- 134.1 (S.D.) mu/ml) returned to values within the normal range (13.86 +/- 5.55) on day 3 after birth, suggesting the utility of the RIA in elucidating the role of hypoxia and/or insulin in increased erythropoiesis in IDM. The serum Ep titers in patients with anemias and polycythemias were compared to those of normal human subjects and agreed well with pathophysiologic mechanisms of these hemopoietic disorders, confirming the validity of the RIA.

• Research Organization: Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA
• OSTI ID: 6377731
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 100:6; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANEMIAS
BETA DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BLOOD FORMATION
BLOOD SERUM
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROPOIESIS
ERYTHROPOIETIN
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
PATHOLOGY
PATIENTS
POLYCYTHEMIA
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SYMPTOMS
TRACER TECHNIQUES
USES