Damage and Recovery of Mouse Testis after 1000 r Acute Localized X-Irradiation, with Reference to Restitution Cells, Sertoli Cell Increase, and Type A Spermatogonial Recovery
After 1000 r of acute localized irradiation many spermatocytes are blocked in first metaphase. Many restitution nuclei similar to those seen after colchicine treatment form between the first and the tenth day after irradiation, the maximum being observed at 6 days. These restituted cells often drift toward the basement membrane. In agreement with previous investigators we have seen an excess number of Sertoli cells in heavily damaged tubules. This excess is not explained by cell division as shown by autoradiography, nor by shrinkage of tubules, since the number is abnormally high even after correction for the shrinkage. A major part of this excess apparently corresponds to the number of restitution cells formed from the blocked first metaphases. The ultimate fate of these indifferent "Sertoli"-like cells is unknown. We have not observed them entering a new spermatogenic cycle. Type A sper- matogonia were observed between 8 and 10 days after 1000 r with a frequency of 0.007 per tubule. After division, the resulting daughter cells distribute themselves along the basement membrane, soon forming a complete ring of dividing spermatogenic cells, observed at 20 days in recovering tubules. Stage "XII" was observed at 5 weeks showing development only through first metaphase spermatocytes. The present findings appear to explain the discrepancies introduced by workers in the 1920's and not resolved since.
- Research Organization:
- Argonne National Lab., Ill.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-14-016566
- OSTI ID:
- 4153059
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 6 Vol. 12; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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