COMPARATIVE EFFECTS OF ELEVATED TEMPERATURE AND 1000 r X-RAYS ON THE SEMINAL EPITHELIUM OF THE MOUSE

Journal Article · Wed Jun 01 04:00:00 EDT 1960 · Cytologia (Tokyo)

DOI:https://doi.org/10.1508/cytologia.25.244· OSTI ID:4087802

Nebel, B R; Murphy, C J

C57 black male mice about 100 days of age were exposed to elevated temperature of 35 deg C and 70% humidity in four exposure periods totaling 61 hr distributed over 4 days. Animals were sacrificed at 3, 5, 7, and 10 days atfer the end of the heat treatment. Results of the heat treatment were compared with those following 1000 r x irradiation. The damage inflicted on the mouse testis by this heat treatment differed fundamentally from that induced by 1000-r acute localized x irradiation. Comparison at 10 days after treatment was especially revealing. Elevated temperature induced the formation of giant cells, originating either from karyokinesis without cytokinesis or from agglomeration. The latter condition was most predominant when spermatozoal heads with indistinct tails were involved. No giant cells were seen after x irradiation. Spermatogonia sur vived the heat treatment so that after 10 days recovery was in full swing. X rays killed spermatogonia with the exception of occasional type A cells. Recovery had not started after 10 days. Late spermatocytes became pycnotic as a result of heat treatment. Of these, some regressed and a few underwent restitution or C-division. Normal spermatids were not formed. Late spermatocytes were blocked by 1000-r x irradiation. The block was so persistent and so severe that 76% of the tubular cross sections seen at 10 days contained some form of restituted or regressed spermatocytes. Ten days after heat treatment no restituted or regressed spermatocytes were seen. This may be related to the fact that heat seems to be less damaging than x rays to the emptying mechanism of tubules. Spermatids appear equally damaged by heat and by x rays. Spermatozoal heads are more common at 10 days after x irradiation than after heat treatment. This may result from the lesser effect of heat on the emptying mechanism as well as from a qualitative difference in the damage inflicted. (auth)

• Research Organization: Argonne National Lab., Ill.
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-010720
• OSTI ID: 4087802
• Journal Information: Cytologia (Tokyo), Journal Name: Cytologia (Tokyo) Vol. Vol: 25
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
CYTOLOGY
GONADS
HUMIDITY
MICE
MITOSIS
RADIATION EFFECTS
RADIATION INJURIES
RECOVERY
TEMPERATURE
TISSUES
X RADIATION