Autoradiographic Study with Tritiated Thymidine of Mouse Testis after 320 r and after 1000 r of Acute Localized X-Irradiation

Journal Article · Fri Jul 01 04:00:00 EDT 1960 · Radiation Research

DOI:https://doi.org/10.2307/3570878· OSTI ID:4199182

Nebel, Bernard R.; Murphy, Carol J.; Linder, Harris J.

Two groups of C57 black male mice approximately 100 days old were subjected to 320-r and 1000-r doses of acute localized x irradiation, respectively. The 320-r series was injected with tritiated thymidine 10 to 15 minutes before exposure; the 1000-r series, 24 hours previously. The distribution of stages showing labeled cells was compared in 320-r and control material and found to be synchronous in both series. After 320 r the respective range of stages at each sacrifice date is narrower than in the control, owing to selective killing of type B spermatogonia. Thus a narrower stream of labeled cells is fed into early meiosis. With 1000 r, synchrony also exists as long as cells are present to maintain it. Cell death is not limited to spermatogonia. After 320 r there is also some loss of spermatocytes and a severe loss of spermatids over and beyond the loss resulting from diminished recruitment. The thymidine label made it possible to trace labeled restitution cells from their original position in the tubule to the basement membrane, but the present study allows no statement concerning their life span and uitimate fate.

• Research Organization: Argonne National Lab., Ill.
• Sponsoring Organization: USDOE
• NSA Number: NSA-14-017759
• OSTI ID: 4199182
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 13; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AGE
ANIMAL CELLS
BIOLOGY AND MEDICINE
GONADS
LABELLED COMPOUNDS
MICE
MITOSIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION DOSES
RADIOAUTOGRAPHY
STANDARDS
THYMIDINE
TRACER TECHNIQUES
TRITIUM
X RADIATION