FACTORS IN THE INACTIVATION OF T2 BACTERIOPHAGE AND MONO-COMPLEX BY IONIZING RADIATIONS. I. THE EFFECTS OF OXYGEN

Journal Article · Sat Oct 01 04:00:00 EDT 1960 · Intern. J. Radiation Biol.

OSTI ID:4135900

Howard-Flanders, P; Jockey, P

The rate of inactivation of T2 phage and the T2 E. coli B mono-complex by 50 kv x ray or by gamma rays was investigated. The phage was protected from indirected radiation were carried out in the presence or in the absence of oxygen. lt was found that oxygen had very little effect on the inactivation of the phage, but that the presence of oxygen doubled the rate of inactivation of the complex. This change in the oxygen effect appears to occur as soon as the phage-DNA enters the bacterial cytoplasm and is independent of whether or not cyanide is added before adsorption. The results are discussed in terms of the reactions of a radiation-induced carbon radical in the phage-DNA which may combine with molecular oxygen and become inactivated, react with a cytoplasmic hydrogenatom donor and regain the active configuration, or become inactivated by a third unknown process if neither oxygen nor a hydrogen donor is present. (auth)

• Research Organization: Univ. of California, Berkeley
• NSA Number: NSA-15-001267
• OSTI ID: 4135900
• Journal Information: Intern. J. Radiation Biol., Journal Name: Intern. J. Radiation Biol. Vol. Vol: 2
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BACTERIOPHAGES
BIOLOGY AND MEDICINE
CARBON
CYANIDES
CYTOLOGY
GAMMA RADIATION
HYDROGEN
NUCLEIC ACIDS
OXYGEN
RADIATION EFFECTS
UREA
X RADIATION