FUNCTIONAL INTERDEPENDENCE OF GENES IN BACTERIOPHAGE T2
Journal Article
·
· Journal of Molecular Biology (England)
Results of experiments on bacteriophages show that the capacity of the cell infected with P/sup 32/-labeled phage to form some of the early proteins is strongly affected by decay of the incorporated isotope. Thus P/sup 32/-labeled T2 phage complexed with bacteria and then frozen loses its ability to make the phage-initiated enzymes, deoxycytidine 5'-phosphate hydroxymethylase and hydroxymethyldeoxycytidine 5'-phosphate kinase, at a rate of 40 to 50% of the rate of loss of infective centers. This suggests that a very large subunit of phage DNA is involved in the synthesis of the enzymes. The fact that the enzyme- forming capacity declined at about 40 to 50% of the rate of infective centers was interpreted as follows. In T2 phage, there are possibly 2 (or 3) functional systems and the loss of infective centers could result from inactivation of any of these functional systems, whereas the loss of enzyme-forming capacity may be due to the inactivation of only 1 of the functional systems. Furthermore, this idea presupposes an all-or-none inactivation of a functional system. This interpretation is compatible with the previous observation that the genetic markers of T2 and T4 phages are located on 1 continuous genetic map. If the genes for the early enzymes also are on this continuous map, 2 (or 3) segments corresponding to the functional systems may be visualized. (TCO)
- Research Organization:
- Univ. of Michigan, Ann Arbor
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-017855
- OSTI ID:
- 4737124
- Journal Information:
- Journal of Molecular Biology (England), Journal Name: Journal of Molecular Biology (England) Vol. Vol: 5; ISSN JMOBA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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