THE PROBLEM OF INTERMEDIATE NUCLEOTIDE METABOLISM IN NORMAL AND X-RAY IRRADIATED BONE MARROW
BS>In rabbit marrow were found adenylic, inosinic, guanylic, and uridylic acids, hypoxanthine, uracil, and other lowmolecular-weight purine and pyrimidine substances. The content of these substances is sharply decreased in two days after local x-ray irradiation at a dose of 2000 r. Also found were traces of low-molecular-weight preformed deoxyribose compounds. Pyrophosphate is completely absent. This shows that the reaction of pyrophosphorolysis is practically absent in bone marrow under natural condi tions. The autolytic splitting of RNA preformed in bone marrow is two to three times faster in onthophosphate buffer solution than in pyrophosphate. This shows the possible presence of a reaction of onthophosphorolysis of RNA along with hydrolytic splitting of RNA. The enzymatic splitting of RNA preformed in bone marrow in ortho and pyrophosphate buffers two days after local irradiation nt a dose of 1000 r is decreased two- to three-fold. The Barton method for determination of deoxyribose compounds was modified as follows: the solution to be analyzed is first brominated by the method of Massant and Hoste. This permits determination by the Banton method not only of purine deoxyribose compounds but also of pyrimidine deoxyribose compounds. (auth)
- Research Organization:
- A.N. Bakh Inst. of Biochemistry, Academy of Sciences, Moscow
- NSA Number:
- NSA-15-000070
- OSTI ID:
- 4126630
- Journal Information:
- Biochemistry (U.S.S.R.) (English Translation), Journal Name: Biochemistry (U.S.S.R.) (English Translation) Vol. Vol: 25
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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