INTERMEDIATE NUCLEOTIDE METABOLISM IN BONE MARROW IN NORMAL SUBJECTS AND AFTER X-RAY IRRADIATION
The low molecular weight purine and pyrimidine compounds in bone marrow before and after irradiation were separated into eight fractions; we established the positions of these fractions under definite conditions of paper chromatography and also determined spectrophotometrically the content of these fractions. We showed in parallel chromatograms with definite nucleotides that the sixth fractions which was present in the greatest amount of all the low molecular purine and pyrimidine compounds was adenylic acid. The content of low molecular weight purine and pyrimidine fractions of bone marrow decreased in two days after localized x-ray irradiation with a dose of 2000 r. The total amount of the studied fractions in bone marrow of an irradiated leg decreased by about 60% compared to an unirradiated leg of the same animal. We have suggested possible reasons for the decreased content of nucleotides in bone marrow after irradiation and the physiological significance of this. Two days after localized x-ray irradiation of one leg of a rabbit with a dose of 2000 r, the enzymatic activity of the bone marrow of this leg, in splitting off from DNA of purine and pyrimidine compounds which are soluble in 60% alcohol, decreased differently in different buffer solutions: in tris buffer solution by 43%, in orthophogphate buffer solution, by 20%. (auth)
- Research Organization:
- A. N. Bakh Inst. of Biochemistry, Academy of Sciences, Moscow
- NSA Number:
- NSA-13-021850
- OSTI ID:
- 4234561
- Journal Information:
- Biochemistry (USSR) (English Translation), Journal Name: Biochemistry (USSR) (English Translation) Vol. Vol: 24
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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NUCLEOTIDE METABOLISM OF GALLFRIA MELLONELLA L CATERPILLARS, NORMAL AND AFTER X-RAY IRRADIATION
ON INTERMEDIATE NUCLEOTIDE METABOLISM IN THE NORM AND RADIATION DISEASE
Related Subjects
ADENOSINE
ADENYLIC ACID
ALCOHOLS
ANIMALS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BONE MARROW
CHROMATOGRAPHY
DNA
ENZYMES
HETEROCYCLICS
METABOLISM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHATES
PHOTOMETRY
PHYSIOLOGY
PURINES
PYRIMIDINES
RABBITS
RADIATION DOSES
RADIATION EFFECTS
SOLUTIONS
SPECTROSCOPY
X RADIATION