PREPARATION OF BILIRUBIN LABELED WITH I$sup 13$$sup 1$

Journal Article · Sun Dec 01 04:00:00 EST 1963 · Intern. J. Appl. Radiation Isotopes

DOI:https://doi.org/10.1016/0020-708X(63)90055-3· OSTI ID:4115335

Anghileri, L J

Iodination of bilirubin was carried out using I/sup 131/. Both iodinated bilirubin and biliverdin were obtained, yields ranged from 7-- 16 percent and 10-- 25 percent, respectively. The purity of each radiocompound was determined chromatographically. Normal human serum albumin labeled with radioiodinated bilirubin was prepared and lyophilized. The bilirubin-albumin complex was assayed 24, 48, and 72 hours after preparation. The final product of bilirubin iodination by this technique is a mixture of bilirubin derivatives, one of which is biliverdin. Separation provides a labeled bilirubin that resembles uniodinated bilirubin in physical, chemical, and physiologic characteristics. The method is a relatively inexpensive way of preparing bile pigments that may be used in bilirubin metabolism studies and in liver function tests. (H.M.G.)

• Research Organization: Volk Radiochemical Co., Skokie, Ill.
• NSA Number: NSA-18-012182
• OSTI ID: 4115335
• Journal Information: Intern. J. Appl. Radiation Isotopes, Journal Name: Intern. J. Appl. Radiation Isotopes Vol. Vol: 14
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALBUMINS
BILIRUBIN
BILIVERDIN
BIOCHEMISTRY
BIOLOGY
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
COLOR
COMPLEXES
ECONOMICS
EFFICIENCY
EVAPORATION
IMPURITIES
IODINE 131
LABELLED COMPOUNDS
LIVER
OPERATION
ORGANIC IODINE COMPOUNDS
PERFORMANCE
PIGMENTS
PREPARATION
QUANTITATIVE ANALYSIS
RADIOCHEMISTRY
SEPARATION PROCESSES
USES