HISTOCHEMICAL EXAMINATIONS OF THE BEHAVIOR OF SOME ENZYMES IN THE LUNG IN ACUTE RADIATION SICKNESS AS WELL AS AFTER LOCAL X IRRADIATION

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Radiobiol. Radiotherap.

OSTI ID:4036920

Kosmider, St; Jonek, J

Histochemical examinations of localization and activity of DPNH- diaphorase, succinic acid dehydrogenase, ATPase, aikaiine and acid phosphatase, and nonspecific as well as E-600-resistant esterase were performed in the lungs of guinea pigs at various stages of acute radiation sickness and after local irradiation of the lungs. The radiation syndrome was caused by a single exposure of the whole animal body to an x-ray dose of 400 r. Pulmonary tissue was x irradiated with 400 r. Detection of enzymes was done 24 hr, 4 and 11 days after whole-body irradiation and 4 days after local irradiation. Results show a decrease of respiratory ferments in the lung after whole-body as well as local irradiation. This may be significant for the oxidationprocesses. ATP-ase and alkaline phosphatase decreased with the two types of irradiation and this may be related to disturbance s in the active substratum transport from blood to cytoplasm and the reverse. The reaction to acid phosphatase showed an increasing tendency, the cause of which was a quantitative increase of the phagocytal cells of the lung, which contain this enzyme in abundance. Thiolacetesterase decrease could be causally connected with disturbances oi the fat metabolism. A quantitative increase of E -600-resistant esterase is explained with intensified protcolytic processes. (auth)

• Research Organization: Klinik fur Innere und Berufskrankheiten der Medizinischen, Akademie, Zabrze, Poland; and Histologische Institut der Medizinischen Akademie, Zabrze, Poland
• NSA Number: NSA-18-019675
• OSTI ID: 4036920
• Journal Information: Radiobiol. Radiotherap., Journal Name: Radiobiol. Radiotherap. Vol. Vol: 4
• Country of Publication: Country unknown/Code not available
• Language: German

Related Subjects

ATP-ASE
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BODY
CYTOLOGY
DEHYDROGENASES
DIAPHORASES
DPNH
ENZYMES
ESTERASES
GUINEA PIGS
IRRADIATION
LIPIDS
LUNGS
METABOLISM
ORGANIC PHOSPHORUS COMPOUNDS
OXIDATION
PHOSPHATASES
PYRIDINES
QUALITATIVE ANALYSIS
RADIATION DOSES
RADIATION SICKNESS
SUCCINIC ACID
TISSUES
VARIATIONS
X RADIATION